Flavin Mononucleotide-Binding Flavoprotein Family in the Domain Archaea
نویسندگان
چکیده
منابع مشابه
Flavin mononucleotide-binding flavoprotein family in the domain Archaea.
The protein (AfpA, for archaeoflavoprotein) encoded by AF1518 in the genome of Archaeoglobus fulgidus was produced in Escherichia coli and characterized. AfpA was found to be a homodimer with a native molecular mass of 43 kDa and containing two noncovalently bound flavin mononucleotides (FMNs). The cell extract of A. fulgidus catalyzed the CO-dependent reduction of AfpA that was stimulated by t...
متن کاملStudies on the binding and function of flavin phosphates with flavin mononucleotide-dependent enzymes.
The binding and function of FMN, 3,6,7-trimethyl-9(l’-o-ribityl)isoahoxazine 5’-phosphate (3-methyl-FMN), 6,7dichloro-9-(l’-o-ribityl)isoalloxazine 5’-phosphate (6,7-dichloro-FMN), and 6,7-dimethyl-9-(5’-hydroxypentyl)isoalloxazine 5’-phosphate (5’-hydroxypentyl-FMN) has been investigated with reduced nicotinamide adenine dinucleotide phosphate dehydrogenase and NADPH cytochrome c reductase fro...
متن کاملFMN - Binding Fluorescent Proteins ( FbFPs ) ▶ Flavin Mononucleotide - Binding Fluorescent Proteins Focused Ion Beam - SEM ▶
reaction coordinate, but as a second dimension the distance between ions needs to be taken into account as ions do not move independently through the pore. The biasing potential in both equilibrium and nonequilibrium methods can be chosen arbitrarily, rendering the PMF approach suitable also for high barriers that are precluded from direct flux estimates, as the spontaneous permeation rate woul...
متن کاملFlavin mononucleotide: the coenzyme of reduced diphosphopyridine nucleotide dehydrogenase.
From the particulate reduced nicotinamide adenine dinucleotide (DPNH) oxidase of beef heart, Mackler (3) has obtained a soluble DPNH dehydrogenase which was estimated to be approximately 90 to 95% homogenous when examined electrophoretically.l Similarly, when the enzyme was chromatographed on diethylaminoethyl cellulose with phosphate buffer, pH 7.5, in a gradient elution method (0.001 M to 0.0...
متن کاملFlavin Mononucleotide: the Acid-extractable Flavin of the Dpnh-coenzyme Q Reductase of the Respiratory Chain.
Several D P N H dehydrogenases have been purified from heart mitochondria or heart-muscle preparationsl-L STRAUB'S enzym& has been shown by MASSEY 8 to be a form of lipoamide dehydrogenase (EC 1.6.4.3), while the relationship of the remaining preparations to the respiratory chain and to each other is obscure. The flavin extractable by acid t reatment has been reported to be FMN in some casesl, ...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Bacteriology
سال: 2004
ISSN: 0021-9193,1098-5530
DOI: 10.1128/jb.186.1.90-97.2004